Consequently, preparatory to conducting the tests consistently and as instances call for subsequently, inoculate diluted specimens of the material to generally be tested with independent feasible cultures of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Salmonella. This may be performed by including 1 mL of not fewer than 10C

Observe the plate after completion of incubation interval and count the total number of colonies in cfu/gm.for not a lot less than 3 days. Take a look at the streaked surfaces under UV gentle. Examine the plates to find out regardless of whether colonies getting the qualities mentioned in Desk three are present. Validate any suspect colonial advanc